Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol

In this study, it was aimed to immunohistochemically evaluate the possible effect of probiotic supplementation on rats fed a high cholesterol diet mast cell heterogeneity and density in urinary bladder expression chymase, tryptase tumor necrosis factor-alpha (TNF-α) cytokine bladder. Three groups were formed study. For 8 weeks, Group 1 (control group) regular rat diet. Food for 2 (feeding +2% cholesterol) (high cholestrol provided weeks. last four 3 (%2 + Lactobacillus acidophilus) (probiotic administered L. acidophilus probiotics. Levels total (TC) measured by using spectrophotometric autoanalyzer. Serum TC levels remarkably increased group compared (P<0.05). decreased significantly (P <0.05) 2. The number cells control close each other. Only safranin O (SO) (+) group. While there no difference between groups, an increase found chymase declined groups. There significant TNF-α immunoreactivity three conclusion, effects probiotics tryptase, revealed.